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ptripz egfp limd1 construct  (Addgene inc)


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    Structured Review

    Addgene inc ptripz egfp limd1 construct
    Ptripz Egfp Limd1 Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptripz+egfp+limd1+construct/pTRIPZ-EGFP%3ALIMD1+(Plasmid+%23108230)/pmc12637021-305-1-5
    Average 94 stars, based on 1 article reviews
    ptripz egfp limd1 construct - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Construct:

    Article Title: Talin–tensin3 interactions regulate fibrillar adhesion formation and tensin3 phase separation
    Article Snippet: To generate GFP-LIMD1 and mCh-LIMD1, full-length LIMD1 cDNA ( Homo sapiens ) was amplified from pTRIPZ-EGFP:LIMD1 ( ) using the primers listed in and Q5 High-fidelity 2× master mix (NEB). .. The pTRIPZ-EGFP:LIMD1 construct (plasmid #108230; Addgene) was a gift from Kenneth Irvine (Rutgers University, Piscataway, NJ, USA). pEGFP-C1 (Clontech) and pmCherry-C1 (Clontech) were linearized using XhoI (Thermo Fisher Scientific) and HindIII (Thermo Fisher Scientific) enzymes by incubation at 37°C for 60 min. .. The amplified LIMD1 cDNA was assembled with the linearized pEGFP and pmCherry vector using the HiFi DNA assembly kit (NEB).

    Plasmid Preparation:

    Article Title: Talin–tensin3 interactions regulate fibrillar adhesion formation and tensin3 phase separation
    Article Snippet: To generate GFP-LIMD1 and mCh-LIMD1, full-length LIMD1 cDNA ( Homo sapiens ) was amplified from pTRIPZ-EGFP:LIMD1 ( ) using the primers listed in and Q5 High-fidelity 2× master mix (NEB). .. The pTRIPZ-EGFP:LIMD1 construct (plasmid #108230; Addgene) was a gift from Kenneth Irvine (Rutgers University, Piscataway, NJ, USA). pEGFP-C1 (Clontech) and pmCherry-C1 (Clontech) were linearized using XhoI (Thermo Fisher Scientific) and HindIII (Thermo Fisher Scientific) enzymes by incubation at 37°C for 60 min. .. The amplified LIMD1 cDNA was assembled with the linearized pEGFP and pmCherry vector using the HiFi DNA assembly kit (NEB).

    Incubation:

    Article Title: Talin–tensin3 interactions regulate fibrillar adhesion formation and tensin3 phase separation
    Article Snippet: To generate GFP-LIMD1 and mCh-LIMD1, full-length LIMD1 cDNA ( Homo sapiens ) was amplified from pTRIPZ-EGFP:LIMD1 ( ) using the primers listed in and Q5 High-fidelity 2× master mix (NEB). .. The pTRIPZ-EGFP:LIMD1 construct (plasmid #108230; Addgene) was a gift from Kenneth Irvine (Rutgers University, Piscataway, NJ, USA). pEGFP-C1 (Clontech) and pmCherry-C1 (Clontech) were linearized using XhoI (Thermo Fisher Scientific) and HindIII (Thermo Fisher Scientific) enzymes by incubation at 37°C for 60 min. .. The amplified LIMD1 cDNA was assembled with the linearized pEGFP and pmCherry vector using the HiFi DNA assembly kit (NEB).



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    ptripz egfp limd1 construct  (Addgene inc)
    94
    Addgene inc ptripz egfp limd1 construct
    Ptripz Egfp Limd1 Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptripz+egfp+limd1+construct/pTRIPZ-EGFP%3ALIMD1+(Plasmid+%23108230)/pmc12637021-305-1-5
    Average 94 stars, based on 1 article reviews
    ptripz egfp limd1 construct - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

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